AGS

€375.00

Excl. VAT

Item no.	CLS-300408
Manufacturer	CLS Cell Lines Service
Amount	1 cryovial
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	Human (Homo sapiens)
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Available
Manufacturers Category	Gastric cancer cell lines
Tissue	Gastric
Disease	Adenocarcinoma
Age	54 years
Gender	Female
Ethnicity	Caucasian
Morphology	Epithelial-like
Growth Properties	Monolayer, adherent
Biosafety Level	2
Protein Expression	p53 positive
Tumorigenic	Yes, in athymic BALB/c mice
Viruses	This cell line may release Parainfluenzavirus Type 5 (formerly known as Simian Virus 5). The virus interferes with Interferon-signalling within the cell line by degradation of STAT1.
Karyotype	Modal number = 47, range = 39 to 92
Culture Medium	DMEM
Medium Supplements	10% FBS, 2 mM L-glutamine, 4.5 g/L glucose
Doubling Time	24 to 48 hours
Subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
Split Ratio	A ratio of 1:2 to 1:6 is recommended
Seeding Density	1 x 10^4 cells/cm^2 will result in a confluent monolayer within 3to5 days.
Fluid Renewal	2 to 3 times per week
Freeze Medium	CM-1 (Catalog number CLS-800650)
Amelogenin	X, X
NOTE	Deutsch: Universitäre Kunden: Für den Erwerb ist ein Material Transfer Agreement oder eine Limited Use Label License auszufüllen. Kommerzielle Kunden: Für den Erwerb ist ein Material Transfer Agreement oder ein Master Supply Agreement auszufüllen. Nach eingegangener Bestellung werden ihnen alle relevanten Dokumente zugeschickt. English: University Customers: A Material Transfer Agreement or Limited Use Label License must be completed for purchase. Commercial Customers: A Material Transfer Agreement or Master Supply Agreement must be completed for purchase. After the order is received, all relevant documents will be sent to you.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

CLS-300408-datasheet.pdf

Request safety datasheet

Simple Order Order Subscription

Amount: 1 cryovial

Listprice: €375.00

Price: €375.00

available

Delivery expected until 9/4/2025

Quantity:

Compare