HT-29

€540.00

Excl. VAT

Item no.	CLS-300215
Manufacturer	CLS Cell Lines Service
Amount	1 cryovial
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	Human (Homo sapiens)
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	HT 29, HT29
Available
Manufacturers Category	Intestine cancer cell lines
Tissue	Colon
Disease	Adenocarcinoma
Age	44 years
Gender	Female
Ethnicity	Caucasian
Morphology	Epithelial-like
Growth Properties	Adherent
Biosafety Level	1
Receptors Expressed	urokinase receptor(u-PAR), vitamin D (moderate expression), no detectable plasminogen activator activity.
Protein Expression	CEA negative, p53 positive
Antigen Expression	Blood Type A, Rh+, HLA A1, A3, B12, B17, Cw5, CD4 -, cell surface expression of galactose ceramide (a possible alternative receptor for HIV)
Isoenzymes	Me-2, 1, PGM3, 1-2, PGM1, 1-2, ES-D, 1, AK-1, 1, GLO-1, 1-2, G6PD, B, Phenotype Frequency Product: 0.0230
Oncogenes	myc+, ras+, myb+, fos+, sis+, p53+, abl -, ros -, src ?
Tumorigenic	Yes, in nude mice. Forms well differentiated adenocarcinoma consistent with colonic primary (grade I), tumors also form in steroid treated hamsters
Virus Susceptibility	human immunodeficiency virus (HIV, LAV)
Products	Secretory component of IgA, carcinoembryonic antigen (CEA), transforming growth factor beta binding protein, mucin, The p53 antigen is overproduced
Karyotype	The stemline chromosome number is hypertriploid with the 2S component occurring at 2.4%. Seventeen marker chromosomes are found in most metaphases, generally in single copy per chromosome. The marker designations are: M1p-(=t(3p-, ?) with a deleted short arm), t(7q, ?), t(10q, ?), i(13q), 19q+a. M6, ?t(8q, 9q-), ?Xp, M9, 6q+, t(13, ?)a, t(13, ?)b, 19q+b, M14, M15, 15p+, and Xq-. Chromosome 13 is nullisomic and chromosomes 8 and 14 are generally monosomic. No Y chromosome was detected by QM band analysis.
Culture Medium	EMEM
Medium Supplements	10% FBS, 2 mM L-glutamine
Doubling Time	24 hours
Subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
Split Ratio	A ratio of 1:3 to 1:8 is recommended
Seeding Density	3 x 10^4 cells/cm^2
Fluid Renewal	2 to 3 times per week
Freezing Recovery	Slow, the cells need roughly 48 hours to settle and adhere.
Freeze Medium	CM-1 (Catalog number CLS-800650)
Amelogenin	X, X
NOTE	Deutsch: Universitäre Kunden: Für den Erwerb ist ein Material Transfer Agreement oder eine Limited Use Label License auszufüllen. Kommerzielle Kunden: Für den Erwerb ist ein Material Transfer Agreement oder ein Master Supply Agreement auszufüllen. Nach eingegangener Bestellung werden ihnen alle relevanten Dokumente zugeschickt. English: University Customers: A Material Transfer Agreement or Limited Use Label License must be completed for purchase. Commercial Customers: A Material Transfer Agreement or Master Supply Agreement must be completed for purchase. After the order is received, all relevant documents will be sent to you.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 cryovial

Listprice: €540.00

Price: €540.00

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Delivery expected until 9/4/2025

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