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NCI-H146

NCI-H146

€375.00

Excl. VAT

Item no.	CLS-300182
Manufacturer	CLS Cell Lines Service
Amount	1 cryovial
Category	Cell Lines & Cell Culture Cell Lines
Type	Cell line
Specific against	Human (Homo sapiens)
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	H146, H-146, NCIH146
Available
Manufacturers Category	Lung cancer cell lines
Tissue	Lung
Disease	Small cell carcinoma
Metastatic Site	Bone marrow
Age	59 years
Gender	Male
Ethnicity	Caucasian
Morphology	Epithelial-like
Growth Properties	Aggregates in suspension
Biosafety Level	1
Receptors Expressed	insulin-like growth factor II receptor (IGF II)
Protein Expression	The cells stain positively for vimentin and keratin, but are negative for neurofilament triplet protein.
Antigen Expression	The line expresses elevated levels of four biochemical markers: neuron specific enolase, brain isoenzyme of creatine kinase, L-DOPA decarboxylase and bombesin-like immunoreactivity
Isoenzymes	G6PD, B, PGM1, 1-2, PGM3, 1-2, ES-D, 1, Me-2, 2, AK-1, 1, GLO-1, 1, Phenotype Frequency Product = 0.0009
Tumorigenic	Forms transplantable tumors in nude mice which histologically resemble tumor cells from the original biopsy specimen
Products	The cells produce relatively high amounts of c-myc mRNA, but c-myc DNA sequences are not amplified. The cells do not express vasopressin, oxytocin or gastrin releasing peptide.
Ploidy Status	Aneuploid
Msi Status	Stable (MSS)
Karyotype	This is a near triploid human cell line. The modal chromosome number is 68, but cells with 66, 70 and 71 chromosomes also occurred frequently. The X chromosomes were paired, and no Y chromosome was detected in QM stained preparations.
Culture Medium	RPMI 1640
Medium Supplements	10% FBS, 2 mM L-glutamine
Subculturing	The cells should be subcultured by transferring part of the suspension into fresh new cell culture flasks prefilled with fresh medium. Alternatively, the clusters may be collected by centrifugation and resuspended in fresh medium.
Split Ratio	A ratio of 1:2 to 1:6 is recommended
Seeding Density	1 to 2 x 10^5 cells/ml
Fluid Renewal	2 to 3 times per week
Freezing Recovery	After thawing allow the cells to recover from the freezing process for at least 24 to 48 hours.
Freeze Medium	CM-1 (Catalog number CLS-800100)
Amelogenin	X, X
NOTE	Deutsch: Universitäre Kunden: Für den Erwerb ist ein Material Transfer Agreement oder eine Limited Use Label License auszufüllen. Kommerzielle Kunden: Für den Erwerb ist ein Material Transfer Agreement oder ein Master Supply Agreement auszufüllen. Nach eingegangener Bestellung werden ihnen alle relevanten Dokumente zugeschickt. English: University Customers: A Material Transfer Agreement or Limited Use Label License must be completed for purchase. Commercial Customers: A Material Transfer Agreement or Master Supply Agreement must be completed for purchase. After the order is received, all relevant documents will be sent to you.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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CLS-300182-datasheet.pdf

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Amount: 1 cryovial

Listprice: €375.00

Price: €375.00

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Delivery expected until 9/4/2025

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