NCI-H146

375,00 €

Zzgl. MwSt.

ArtNr	CLS-300182
Hersteller	CLS Cell Lines Service
Menge	1 cryovial
Kategorie	Cell Lines & Cell Culture Cell Lines
Typ	Cell line
Specific against	Human (Homo sapiens)
Dry ice	Yes
ECLASS 10.1	42040401
ECLASS 11.0	42040401
UNSPSC	41106509
Alias	H146, H-146, NCIH146
Lieferbar
Manufacturers Category	Lung cancer cell lines
Tissue	Lung
Disease	Small cell carcinoma
Metastatic Site	Bone marrow
Age	59 years
Gender	Male
Ethnicity	Caucasian
Morphology	Epithelial-like
Growth Properties	Aggregates in suspension
Biosafety Level	1
Receptors Expressed	insulin-like growth factor II receptor (IGF II)
Protein Expression	The cells stain positively for vimentin and keratin, but are negative for neurofilament triplet protein.
Antigen Expression	The line expresses elevated levels of four biochemical markers: neuron specific enolase, brain isoenzyme of creatine kinase, L-DOPA decarboxylase and bombesin-like immunoreactivity
Isoenzymes	G6PD, B, PGM1, 1-2, PGM3, 1-2, ES-D, 1, Me-2, 2, AK-1, 1, GLO-1, 1, Phenotype Frequency Product = 0.0009
Tumorigenic	Forms transplantable tumors in nude mice which histologically resemble tumor cells from the original biopsy specimen
Products	The cells produce relatively high amounts of c-myc mRNA, but c-myc DNA sequences are not amplified. The cells do not express vasopressin, oxytocin or gastrin releasing peptide.
Ploidy Status	Aneuploid
Msi Status	Stable (MSS)
Karyotype	This is a near triploid human cell line. The modal chromosome number is 68, but cells with 66, 70 and 71 chromosomes also occurred frequently. The X chromosomes were paired, and no Y chromosome was detected in QM stained preparations.
Culture Medium	RPMI 1640
Medium Supplements	10% FBS, 2 mM L-glutamine
Subculturing	The cells should be subcultured by transferring part of the suspension into fresh new cell culture flasks prefilled with fresh medium. Alternatively, the clusters may be collected by centrifugation and resuspended in fresh medium.
Split Ratio	A ratio of 1:2 to 1:6 is recommended
Seeding Density	1 to 2 x 10^5 cells/ml
Fluid Renewal	2 to 3 times per week
Freezing Recovery	After thawing allow the cells to recover from the freezing process for at least 24 to 48 hours.
Freeze Medium	CM-1 (Catalog number CLS-800100)
Amelogenin	X, X
NOTE	Deutsch: Universitäre Kunden: Für den Erwerb ist ein Material Transfer Agreement oder eine Limited Use Label License auszufüllen. Kommerzielle Kunden: Für den Erwerb ist ein Material Transfer Agreement oder ein Master Supply Agreement auszufüllen. Nach eingegangener Bestellung werden ihnen alle relevanten Dokumente zugeschickt. English: University Customers: A Material Transfer Agreement or Limited Use Label License must be completed for purchase. Commercial Customers: A Material Transfer Agreement or Master Supply Agreement must be completed for purchase. After the order is received, all relevant documents will be sent to you.

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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CLS-300182-datasheet.pdf

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Einfache Bestellung Periodische Bestellung

Menge: 1 cryovial

Listenpreis: 375,00 €

Preis: 375,00 €

lieferbar

Lieferung vsl. bis 04.09.2025

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