Comparison

SARS-CoV-2 (COVID-19) ORF9c Antibody

€159.00
Excl. VAT
Item no. PRS-9291-0.02mg
Manufacturer ProSci
Amount 0.02 mg
Category
Type Antibody Polyclonal
Format Liquid
Applications WB, ELISA
Specific against other
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias ORF9c protein, Uncharacterized protein 14, ORF14, ORF9c
Available
Shipping
blue ice or RT
By Research Area
Infectious Disease, COVID-19
Immunogen
Anti-SARS-CoV-2 (COVID-19) ORF9c antibody (9291) was raised against a peptide corresponding to 16 amino acids near the amino-terminus of SARS-CoV-2 (COVID-19) ORF9c protein.

The immunogen is located within the first 50 amino acids of the SARS-CoV-2 (COVID-19) ORF9c protein.
Applications
WB: 0.25-1 μ g/mL

Antibody validated: SARS-CoV-2 (COVID-19) ORF9c antibody can detect 2 ng of free peptide at 1 μ g/mL in ELISA. It can detect SARS-CoV-2 ORF9c in transfected human cells by WB. All other applications and species not yet tested
Specificity
ORF9c Antibody is predicted to not cross-react with other coronavirus family members.
Predicted Molecular Weight
8kD
Purification
SARS-CoV-2 (COVID-19) ORF9c Antibody is affinity chromatography purified via peptide column.
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Buffer
SARS-CoV-2 (COVID-19) ORF9c antibody is supplied in PBS containing 0.02% sodium azide.
Concentration
1 mg/mL
Storage Conditions
SARS-CoV-2 (COVID-19) ORF9c antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Related Products
Cat. No. 9291P - SARS-CoV-2 (COVID-19) ORF9c Peptide
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Ncbi Official Symbol
ORF9c
Accession #
P0DTD3
Protein Gi #
1835922084
User Note
Optimal dilutions for each application to be determined by the researcher.
Ncbi Organism
Severe acute respiratory syndrome coronavirus 2
Swissprot #
P0DTD3
Background
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019–20 coronavirus outbreak (2). SARS-CoV-2 virus proteins include structural proteins, non-structural proteins and accessory factors. The structure of SARS-CoV-2 consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. SARS-CoV-2 non-structural protein is ORF1ab that consists of 16 proteins (nsp1-nsp16), while accessory factors include ORF3a, ORF3b, ORF6, ORF7a, ORF7b, ORF8, ORF9b, ORF9c and ORF10. ORF9c may play a role in host-virus interaction.
Background References 1
Gorbalenya. bioRxiv: 2020.
Background References 2
Hui et al. Int J Infect Dis. 2020; 91:264-266.
1st Image Caption
Figure 1 Western Blot Validation with SARS-CoV-2 (COVID-19) ORF9c Overexpressed 293 Cells
Loading: 10 μ g per lane of 293 cell lysate from WT and SARS-CoV-2 (COVID-19) ORF9c transfected cells.Antibodies: SARS-CoV-2 (COVID-19) ORF9c 9291, 1 μ g/ml, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
2nd Image Caption
Figure 2 Western Blot Validation with SARS-CoV-2 (COVID-19) ORF9c Overexpressed 293 Cells
Loading: 10 μ g per lane of 293 cell lysate from WT and SARS-CoV-2 (COVID-19) ORF9c transfected cells.Antibodies: SARS-CoV-2 (COVID-19) ORF9c, 9291, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 0.25 μ g/mL and Lane 2: 0.5 μ g/mL
3rd Image Caption
Figure 3 ELISA Validation
Antibodies: SARS-CoV-2 (COVID-19) ORF9c Antibody, 9291. A direct ELISA was performed using SARS-CoV-2 ORF9c immunogen peptide (9291P)) as coating antigen and the anti-SARS-CoV-2 (COVID-19) ORF9c antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 0.02 mg
Available: In stock
Listprice: €159.00
Price: €159.00
available

Delivery expected until 10/2/2025 

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