SARS-CoV-2 (COVID-19) ORF9c Antibody

Infectious Disease, COVID-19

WB: 0.25-1 μ g/mL

Antibody validated: SARS-CoV-2 (COVID-19) ORF9c antibody can detect 2 ng of free peptide at 1 μ g/mL in ELISA. It can detect SARS-CoV-2 ORF9c in transfected human cells by WB. All other applications and species not yet tested

8kD

Polyclonal

Unconjugated

1 mg/mL

Cat. No. 9291P - SARS-CoV-2 (COVID-19) ORF9c Peptide

ORF9c

1835922084

Severe acute respiratory syndrome coronavirus 2

Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019–20 coronavirus outbreak (2). SARS-CoV-2 virus proteins include structural proteins, non-structural proteins and accessory factors. The structure of SARS-CoV-2 consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. SARS-CoV-2 non-structural protein is ORF1ab that consists of 16 proteins (nsp1-nsp16), while accessory factors include ORF3a, ORF3b, ORF6, ORF7a, ORF7b, ORF8, ORF9b, ORF9c and ORF10. ORF9c may play a role in host-virus interaction.

Hui et al. Int J Infect Dis. 2020; 91:264-266.

Figure 2 Western Blot Validation with SARS-CoV-2 (COVID-19) ORF9c Overexpressed 293 Cells
Loading: 10 μ g per lane of 293 cell lysate from WT and SARS-CoV-2 (COVID-19) ORF9c transfected cells.Antibodies: SARS-CoV-2 (COVID-19) ORF9c, 9291, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 0.25 μ g/mL and Lane 2: 0.5 μ g/mL