Vimentin Antibody

Cancer, Cell Cycle, Neuroscience, Signal Transduction

This Vimentin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-90 amino acids from human Vimentin.

54 kDa

Polyclonal

Unconjugated

batch dependent

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

VIM

55977767

Optimal dilutions for each application to be determined by the researcher.

Vimentin

P08670

References for protein:

Garcia-Verdugo, I., Biochemistry 47 (18), 5127-5138 (2008)

Fluorescent confocal image of SY5Y cells stained with Vimentin (S82) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with Vimentin (S82) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the Vimentin immunosignal to the cytoskeleton, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000026025).

Western blot analysis of VIM using rabbit polyclonal Vimentin Antibody (S82) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the VIM gene (Lane 2).