Vimentin Antibody

684,00 €

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ArtNr	PRS-61-468-400ul
Hersteller	ProSci
Menge	400 ul
Kategorie	Neuroscience Neural Lineage Markers Molecular Targets for Neuroscience Neural Development Antibodies Cell Biology Cytoskeleton Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies Intermediate filaments Stem Cell Markers
Typ	Antibody Polyclonal
Format	Liquid
Applikationen	WB, IF, IHC-P
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus)
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Vimentin, VIM
Lieferbar
Shipping	blue ice or RT
By Research Area	Cancer, Cell Cycle, Neuroscience, Signal Transduction
Homology	Predicted species reactivity based on immunogen sequence: Bovine, Hamster, Monkey, Rat
Immunogen	This Vimentin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-90 amino acids from human Vimentin.
Applications	For IF starting dilution is: 1:200 For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight	54 kDa
Purification	This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality	Polyclonal
Isotype	Rabbit Ig
Conjugate	Unconjugated
Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration	batch dependent
Storage Conditions	Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	VIM
Accession #	P08670
Protein Gi #	55977767
Ncbi Gene Id #	7431
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	VIM
Ncbi Official Full Name	Vimentin
Ncbi Organism	Homo sapiens
Swissprot #	P08670
Background	Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.
Background References 1	References for protein:
Background References 2	Whipple, R.A., Cancer Res. 68 (14), 5678-5688 (2008)
Background References 3	Garcia-Verdugo, I., Biochemistry 47 (18), 5127-5138 (2008)
Background References 4	Merdes, A., J. Cell Biol. 115 (2), 397-410 (1991)
1st Image Caption	Fluorescent confocal image of SY5Y cells stained with Vimentin (S82) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with Vimentin (S82) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the Vimentin immunosignal to the cytoskeleton, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000026025).
2nd Image Caption	Western blot analysis of Vimentin-S82 in Hela cell line lysates (35ug/lane)
3rd Image Caption	Western blot analysis of VIM using rabbit polyclonal Vimentin Antibody (S82) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the VIM gene (Lane 2).
4th Image Caption	Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Vimentin Antibody (S82) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 400 ul

Listenpreis: 684,00 €

Preis: 684,00 €

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