IL-17 Antibody

blue ice or RT

IL-17 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human IL-17A.

The immunogen is located within amino acids 40 - 90 of IL-17.

Cat. No. 1208 - THP-1 Cell Lysate

Predicted: 17.5kD

Observed: 20 kD (Post-modification: 1 N-linked glycosylation)

Human IL-17 has only one isoform (155aa, 17.5kD). Mouse IL-17 has one isoform (158aa, 17.5kD) and rat IL-17 also has one isoform (150aa, 16.9kD). 4877 can detect only human isoform, but cannot detect mouse and rat.

Polyclonal

Unconjugated

1 mg/mL

Cat. No. 4877P – IL-17 Peptide

None

NP_002181

3605

IL17A

Homo sapiens

IL-17 Antibody: Interleukin 17 (IL-17) is a family of pro-inflammatory cytokines produced by activated T cells and is thought to have a major role in the initiation and perpetuation of rheumatoid arthritis. IL-17 regulates the activities of NF-κ B and mitogen-activated protein kinases such as ERK and JNK. In addition, IL-17 stimulates the expression of IL-6 and cyclooxygenase-2 and enhances the production of nitric oxide. IL-17-producing T helper cells (TH-17 cells) have been the subject of much attention due to the importance of IL-17 in the pathogenesis of autoimmune inflammation. Because of its role in autoimmune diseases, it is thought that targeting the production and action of IL-17 would be beneficial therapeutically in these diseases.

Paunovic et al. Rheumatol.2008; 47:771-6.

Figure 1 Western Blot Validation in Human THP-1 Cell Lysate
Loading: 15 μ g of lysates per lane.Antibodies: IL-17 4877 (A: 1 μ g/mL and B: 2 μ g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 3 Recombinant Protein Test
Loading: 30 ng of human IL-17 recombinant protein per lane.Antibodies: IL-17 4877, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 0.125 μ g/mLLane 2: 0.25 μ g/mLLane 3: 0.5 μ g/mL