IL-17 Antibody

159,00 €

Zzgl. MwSt.

ArtNr	PRS-4877-0.02mg
Hersteller	ProSci
Menge	0.02 mg
Kategorie	Neuroscience Neuroinflammation Metabolism Diabetes Glucose Homeostasis Neural Signaling Antibodies Cell Biology Cell Culture Cytokines Western Blot ELISA Primary Antibodies
Typ	Antibody Polyclonal
Format	Liquid
Applikationen	WB, ELISA
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus)
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	IL-17 Antibody: IL17, CTLA8, IL-17, IL-17A, IL17, Interleukin-17A, Cytotoxic T-lymphocyte-associated antigen 8
Lieferbar
Shipping	blue ice or RT
By Research Area	Signal Transduction
Immunogen	IL-17 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human IL-17A. The immunogen is located within amino acids 40 - 90 of IL-17.
Applications	WB: 0.125-4 μ g/mL. Antibody validated: Western Blot in human and mouse samples. All other applications and species not yet tested.
Positive Control 1	Cat. No. 1208 - THP-1 Cell Lysate
Positive Control 2	Cat. No. 1288 - A20 Cell Lysate
Predicted Molecular Weight	Predicted: 17.5kD Observed: 20 kD (Post-modification: 1 N-linked glycosylation)
Validation	Recombinant Protein Test (Figure 3): Anit-IL-17 antibodies (4877) detected human IL-17 recombinant protein at different concentrations.
Isoforms	Human IL-17 has only one isoform (155aa, 17.5kD). Mouse IL-17 has one isoform (158aa, 17.5kD) and rat IL-17 also has one isoform (150aa, 16.9kD). 4877 can detect only human isoform, but cannot detect mouse and rat.
Purification	IL-17 Antibody is affinity chromatography purified via peptide column.
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	IL-17 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Storage Conditions	IL-17 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Related Products	Cat. No. 4877P – IL-17 Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	IL17A
Accession #	NP_002181
Protein Gi #	4504651
Ncbi Gene Id #	3605
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	IL17A
Ncbi Official Full Name	interleukin 17A
Ncbi Organism	Homo sapiens
Swissprot #	Q16552
Background	IL-17 Antibody: Interleukin 17 (IL-17) is a family of pro-inflammatory cytokines produced by activated T cells and is thought to have a major role in the initiation and perpetuation of rheumatoid arthritis. IL-17 regulates the activities of NF-κ B and mitogen-activated protein kinases such as ERK and JNK. In addition, IL-17 stimulates the expression of IL-6 and cyclooxygenase-2 and enhances the production of nitric oxide. IL-17-producing T helper cells (TH-17 cells) have been the subject of much attention due to the importance of IL-17 in the pathogenesis of autoimmune inflammation. Because of its role in autoimmune diseases, it is thought that targeting the production and action of IL-17 would be beneficial therapeutically in these diseases.
Background References 1	Miossec. Curr. Opin. Rheumatol.2000; 12:181-5.
Background References 2	Paunovic et al. Rheumatol.2008; 47:771-6.
Background References 3	Steinman. Nat. Med.2007; 13:139-145.
1st Image Caption	Figure 1 Western Blot Validation in Human THP-1 Cell Lysate Loading: 15 μ g of lysates per lane.Antibodies: IL-17 4877 (A: 1 μ g/mL and B: 2 μ g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
2nd Image Caption	Figure 2 Western Blot Validation in A-20 Cell Lysate Loading: 15 μ g of lysates per lane.Antibodies: IL-17 4877 (A: 2 μ g/mL and B: 4 μ g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
3rd Image Caption	Figure 3 Recombinant Protein Test Loading: 30 ng of human IL-17 recombinant protein per lane.Antibodies: IL-17 4877, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 0.125 μ g/mLLane 2: 0.25 μ g/mLLane 3: 0.5 μ g/mL

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Menge: 0.02 mg

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Preis: 159,00 €

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