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Neuroligin 1 Antibody

Neuroligin 1 Antibody

Item no.	PRS-45-957-0.1mg
Manufacturer	ProSci
Amount	0.1 mg
Category	Neuroscience Neural Lineage Markers Neural Adhesion Molecules Antibodies ELISA Primary Antibodies Adhesion Molecule Markers Schwann Cell Markers
Type	Antibody Primary
Format	Liquid
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	NLGN1, KIAA1070, MGC45115, neuroligin 1, OTTHUMP00000211275, OTTHUMP00000211276
Available
Shipping	blue ice or RT
By Research Area	Cancer
Homology	Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen	The immunogen for this antibody is: C-TAKQDDPKQQ
Applications	Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Preliminary experiments gave an approx 150kDa band in Human Brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 92.0 kDa according to NP_055747.1. The 150kDa band was successfully blocked by incubation with the immunizing peptide.Immunofluorescence: Strong expression of the protein seen in the membranes/ cytoplasm of U251 and U2OS cells. Recommended concentration: 10ug/ml.
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality	Polyclonal
Conjugate	Unconjugated
Buffer	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration	500 ug/mL
Storage Conditions	Aliquot and store at -20˚ C. Minimize freezing and thawing.
Related Products	Cat. No. 45-957P - Neuroligin 1 Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	NLGN1
Accession #	NP_055747.1
Protein Gi #	7662470
Ncbi Gene Id #	22871
Ncbi Official Symbol	NLGN1
Ncbi Official Full Name	neuroligin 1
Ncbi Organism	Homo sapiens
Swissprot #	Q8N2Q7
Background References 1	Chih B, Engelman H, Scheiffele P. Control of Excitatory and Inhibitory Synapse Formation by Neuroligins. Science. 2005 Jan 27;
1st Image Caption	45-957 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane/cytoplasmic staining. The nuclear stain i
2nd Image Caption	45-957 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane/cytoplasmic staining. The nuclear stain i

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.1 mg

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Delivery expected until 8/7/2025

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