Vergleich

Neuroligin 1 Antibody

ArtNr PRS-45-957-0.1mg
Hersteller ProSci
Menge 0.1 mg
Kategorie
Typ Antibody Primary
Format Liquid
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias NLGN1, KIAA1070, MGC45115, neuroligin 1, OTTHUMP00000211275, OTTHUMP00000211276
Lieferbar
Shipping
blue ice or RT
By Research Area
Cancer
Homology
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen
The immunogen for this antibody is: C-TAKQDDPKQQ
Applications
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Preliminary experiments gave an approx 150kDa band in Human Brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 92.0 kDa according to NP_055747.1. The 150kDa band was successfully blocked by incubation with the immunizing peptide.Immunofluorescence: Strong expression of the protein seen in the membranes/ cytoplasm of U251 and U2OS cells. Recommended concentration: 10ug/ml.
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Conjugate
Unconjugated
Buffer
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration
500 ug/mL
Storage Conditions
Aliquot and store at -20˚ C. Minimize freezing and thawing.
Related Products
Cat. No. 45-957P - Neuroligin 1 Peptide
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
NLGN1
Accession #
NP_055747.1
Protein Gi #
7662470
Ncbi Gene Id #
22871
Ncbi Official Symbol
NLGN1
Ncbi Official Full Name
neuroligin 1
Ncbi Organism
Homo sapiens
Swissprot #
Q8N2Q7
Background References 1
Chih B, Engelman H, Scheiffele P. Control of Excitatory and Inhibitory Synapse Formation by Neuroligins. Science. 2005 Jan 27;
1st Image Caption
45-957 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane/cytoplasmic staining. The nuclear stain i
2nd Image Caption
45-957 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane/cytoplasmic staining. The nuclear stain i

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 0.1 mg
Lieferbar: In stock
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Lieferung vsl. bis 07.08.2025 

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