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Vimentin Antibody [VIM66-1]

Vimentin Antibody [VIM66-1]

€788.00

Excl. VAT

Item no.	PRS-33-861-100ug
Manufacturer	ProSci
Amount	100 ug
Category	Oncology Neuroscience Neural Lineage Markers Molecular Targets for Neuroscience Neural Development Antibodies By Cell Type Carcinoma Cell Biology Cytoskeleton Immunohistochemistry Western Blot Primary Antibodies Intermediate filaments Stem Cell Markers
Type	Antibody Monoclonal
Format	Liquid
Applications	WB, IHC
Specific against	Human (Homo sapiens)
Host	Mouse
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	Vimentin, VIM
Available
Shipping	blue ice or RT
By Research Area	Cancer, Cell Cycle, Neuroscience, Signal Transduction
Immunogen	Full length human recombinant protein was used as the immunogen for the Vimentin antibody.
Applications	Western blot: 1-2 ug/ml IHC (FFPE): 1-2 ug/ml for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) Titering of the Vimentin antibody may be required for optimal performance. 1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Purification	Protein G affinity chromatography
Clonality	Monoclonal
Clone	VIM66-1
Isotype	IgG1, k
Conjugate	Unconjugated
Buffer	PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration	0.2 mg/mL
Storage Conditions	Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	VIM
Ncbi Gene Id #	7431
User Note	Optimal dilutions for each application to be determined by the researcher
Ncbi Official Symbol	VIM
Ncbi Official Full Name	vimentin
Ncbi Organism	Homo sapiens
Swissprot #	P08670
Background	Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. It is involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. [UniProt]
1st Image Caption	Western blot testing of Vimentin antibody and Raji cell lysate. Predicted molecular weight ~58 kDa.
2nd Image Caption	FFPE human melanoma stained with Vimentin antibody (VIM66-1).

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ug

Listprice: €788.00

Price: €788.00

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Delivery expected until 9/4/2025

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