Vergleich

Vimentin Antibody [VIM66-1]

788,00 €
Zzgl. MwSt.
ArtNr PRS-33-861-100ug
Hersteller ProSci
Menge 100 ug
Kategorie
Typ Antibody Monoclonal
Format Liquid
Applikationen WB, IHC
Specific against Human (Homo sapiens)
Host Mouse
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias Vimentin, VIM
Lieferbar
Shipping
blue ice or RT
By Research Area
Cancer, Cell Cycle, Neuroscience, Signal Transduction
Immunogen
Full length human recombinant protein was used as the immunogen for the Vimentin antibody.
Applications
Western blot: 1-2 ug/ml

IHC (FFPE): 1-2 ug/ml for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

Titering of the Vimentin antibody may be required for optimal performance.

1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purification
Protein G affinity chromatography
Clonality
Monoclonal
Clone
VIM66-1
Isotype
IgG1, k
Conjugate
Unconjugated
Buffer
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration
0.2 mg/mL
Storage Conditions
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications
None
Ncbi Official Symbol
VIM
Ncbi Gene Id #
7431
User Note
Optimal dilutions for each application to be determined by the researcher
Ncbi Official Symbol
VIM
Ncbi Official Full Name
vimentin
Ncbi Organism
Homo sapiens
Swissprot #
P08670
Background
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. It is involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. [UniProt]
1st Image Caption
Western blot testing of Vimentin antibody and Raji cell lysate. Predicted molecular weight ~58 kDa.
2nd Image Caption
FFPE human melanoma stained with Vimentin antibody (VIM66-1).

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 ug
Lieferbar: In stock
Listenpreis: 788,00 €
Preis: 788,00 €
lieferbar

Lieferung vsl. bis 04.09.2025 

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