Costunolide

Hydron N containing VEGF are implanted into mouse cornea.

(3aS, 6E, 10E, 11aR)-6, 10-dimethyl-3-methylene-3, 3a, 4, 5, 8, 9-hexahydrocyclodeca[b]furan-2(11aH)-one

Costunolide inhibits FPTase with IC50 of 20 uM and also inhibits telomerase with IC50 of 90 uM and 65 uM in MCF-7 and MDA-MB-231 cells.

Costunolide is dissolved in dimethylsulfoxide (DMSO).

Costunolide inhibits the growth and telomerase activity of MCF-7 and MDA-MB-231 cells in a concentration- and time-dependent manner. [1] Costunolide also inhibits the farnesylation process of human lamin-B by farnesyl–proteinttransferase (FPTase), in a dose dependent manner. Continuously treatment of Costunolide for 48 hours will significantly decrease proliferation of human tumor cells (A549, SK-OV-3, SK-MEL-2, XF498 and HCT-1) in a dose-dependent manner. [2] Costunolide induces apoptosis by ROS-mediated mitochondrial permeability transition and cytochrome C release to the cytosol in HL-60 human leukemia cells. [3] A recent study indicates that Costunolide shows significant antifungal activity, including Trichophyton mentagrophytes, T.simlii, T.rubrum, and so on. [4]

48 hours

1) Plate 500-10, 000 cells in 200 uL media per well in a 96 well plate. Leave 8 wells empty for blank controls. 2) Incubate (37 C, 5% CO2) overnight to allow the cells to attach to the wells. 3) Add 2 uL of Costunolide dissolved in DMSO to each well. Place on a shaking table, 150 rpm for 5 minutes, to thoroughly mix the samples into the media., 5) Incubate (37 C, 5% CO2) for 48 hours to allow Costunolide to take effect. 6) Make 2 mL or more of MTT solution per 96 well plate at 5 mg/mL in PBS. Do not make a stock as MTT in solution is not stable long-term. 7) Add 20 uL MTT solution to each well. Place on a shaking table, 150 rpm for 5 minutes, to thoroughly mix the MTT into the media., 8) Incubate (37 C, 5% CO2) for 1-5 hours to allow the MTT to be metabolized. 9) Dump off the media. (Dry plate on paper towels to remove residue if necessary. 10) Resuspend formazan (MTT metabolic product) in 200 uL DMSO. Place on a shaking table, 150 rpm for 5 minutes, to thoroughly mix the formazan into the solvent. 11) Read optical density at 560 nm and subtract background at 670 nm. Optical density should be directly correlated with cell quantity.

Costunolide Chemical Structure

2 years -20CPowder, 2 weeks4Cin DMSO, 2 months-80Cin DMSO