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PPP1CC Antibody, KO Validated

PPP1CC Antibody, KO Validated

522,00 €

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ArtNr	PRS-19-252-50uL
Hersteller	ProSci
Menge	50 uL
Kategorie	Antibodies Immunohistochemistry Western Blot Primary Antibodies CHiP
Typ	Antibody Primary
Format	Liquid
Applikationen	WB, IHC, CHIP
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Serine/threonine-protein phosphatase PP1-gamma catalytic subunit, PP-1G, Protein phosphatase 1C catalytic subunit, PPP1CC
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Shipping	blue ice or RT
By Research Area	Cancer, Cell Cycle, Neuroscience, Signal Transduction
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-323 of human PPP1CC (NP_002701.1).
Applications	WB: 1:200 - 1:2000 IHC: 1:50 - 1:200 CHIP: 1:50 - 1:200
Positive Control 1	293T
Positive Control 2	HL-60
Positive Control 3	Mouse thymus
Positive Control 4	Mouse spleen
Positive Control 5	Rat brain
Predicted Molecular Weight	Observed: 38kDa
Validation	Antibody is Knockout validated.
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	PPP1CC
Ncbi Gene Id #	5501
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	PPP1CC
Ncbi Official Full Name	Serine/threonine-protein phosphatase PP1-gamma catalytic subunit
Ncbi Organism	Homo sapiens
Swissprot #	P36873
Background	The protein encoded by this gene belongs to the protein phosphatase family, PP1 subfamily. PP1 is an ubiquitous serine/threonine phosphatase that regulates many cellular processes, including cell division. It is expressed in mammalian cells as three closely related isoforms, alpha, beta/delta and gamma, which have distinct localization patterns. This gene encodes the gamma isozyme. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
1st Image Caption	Western blot analysis of extracts of various cell lines, using PPP1CC antibody (19-252) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
2nd Image Caption	Western blot analysis of extracts from normal (control) and PPP1CC knockout (KO) HeLa cells, using PPP1CC antibody (19-252) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
3rd Image Caption	Immunohistochemistry of paraffin-embedded human gastric cancer using PPP1CC antibody (19-252) at dilution of 1:100 (40x lens).
4th Image Caption	Immunohistochemistry of paraffin-embedded rat brain using PPP1CC antibody (19-252) at dilution of 1:100 (40x lens).
5th Image Caption	Immunohistochemistry of paraffin-embedded human stomach using PPP1CC antibody (19-252) at dilution of 1:100 (40x lens).

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Menge: 50 uL

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Preis: 522,00 €

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