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The 2.4G2 antibody is specific for a common epitope found in the extracellular regions of mouse Fc-receptors Fc-gamma II (CD32) and Fc-gamma III (CD16). As these are receptors for the Fc portion of mouse IgG, they may also bind laboratory antibody preparations and products used in a variety of cell analysis protocols such as flow cytometry, immunohistochemistry and functional cell assays. The 2.4G2 antibody is therefore widely used as a pre-treatment reagent to block binding of specific antibodies of interest, e.g. fluorescently conjugated antibodies, to Fc receptors via their Fc domains and contributing to " non-specific" staining.
Recent Publications: Shim YR and Lee HK. 2015. Immune Netw. 15(2): 73-82. (Blocking - Flow Cytometry)
Stephen TL, Wilson BS, and Laufer TM. 2012. Proc. Natl. Acad. Sci. 109: 7415-7420. (Blocking- Immunofluorescence microscopy)
Yamaji O, Nagaishi T, Totsuka T, Onizawa M, Suzuki M, Tsuge et al. 2012. J. Immunol. 188:2524-2536. (Blocking - in vitro)
Shimazu T, Iida R, Zhang Q, Welner RS, Medina KL, Alberola-Ila J, and Kincade PW. 2012. Blood. 119:4889-4897. (Blocking - Flow cytometry)
Stoeker L, Nordone S, Gunderson S, Zhang L, Kajikawa A, LaVoy A, Miller M, Klaenhammer TR, and Dean GA. 2011. Clin. Vaccine Immunol. 18: 1834-1844. (Blocking - Flow cytometry)
Beretta F, St.-Pierre J, Piccirillo CA, and Stevenson MM. 2011. J. Immunol. 186: 4862-4871. (Blocking - Flow cytometry)
Coudert JD, Scarpellino L, Gros F, Vivier E, and Held W. 2008 Blood. 111: 3571-3578. (Immunoprecipitation)
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.
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