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The 104 antibody reacts with mouse CD45.2, also known as Ly5.2, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA). Functionally, CD45 is a protein tyrosine phosphatase whose broad cell distribution supports a critical role in many leukocyte functions, including regulation of signal transduction and cell activation associated with the T cell and B cell receptors.
The 104 antibody is typically used as a leukocyte marker in Ly5.2 mouse strains C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A and AKR. The antibody has been demonstrated to be specific for CD45.2 and is not cross-reactive with CD45.1-bearing cells.
Willinger T and Flavell, RA. 2012. Proc. Natl. Acad. Sci. 109: 8670 - 8675. (Flow Cytometry)
Hale JS, Nelson LT, Simmons KB, and Fink PJ. 2011. J. Immunol. 186: 799 - 806. (Flow Cytometry)
Orr MT, Beilke JN, Proekt I, and Lanier LL. 2010. Proc. Natl. Acad. Sci. 107: 15844 - 15849. (Flow Cytometry)
Banerjee K, Biswas PS, Kumaraguru U, Schoenberger SP, and Rouse BT. 2004. 173: 7575-7583. (Immunofluorescence microscopy - frozen tissue)
Favre CJ, Mancuso M, Maas K, McLean JW, Baluk P, and Mcdonald DM. 2003. Am. J. Physiol. Heart Circ. Physiol. 285:H1917-H1938. (Immunocytochemistry)
Shen F-W, Tung J-S, and Boyse EA. 1986. Immunogenetics. 24(3): 146-149. (Immunoprecipitation).
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.
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