Comparison

FITC Anti-Human/Mouse CD45R (B220) (RA3-6B2) European Partner

€43.00
Excl. VAT
Item no. 35-0452-U100
Manufacturer Tonbo Biosciences
Amount 100 ug
Category
Type Antibody Monoclonal
Format FITC
Applications FC
Clone RA3-6B2
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus)
Host Rat
Isotype IgG2a kappa
Conjugate/Tag FITC
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias Ly-5, Lyt-4, T200
Similar products B220
Available
Description

The RA3-6B2 antibody reacts with the human and mouse CD45 isoform known as CD45R, or B220, a protein tyrosine phosphatase of 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns, all critical for leukocyte function. In mouse, the CD45R/B220 isoform is predominantly found on B cells, at varying levels on all stages from pro-B cells to activated B cells, and may also be detected on certain T cell and NK cell subsets. It is of note that B220 is not similarly expressed on human B cells, where it appears to be differentiation-specific and therefore expressed on only some B cell subsets. Other forms of CD45 with restricted cellular expression include CD45RA, CD45RB, CD45RO and several others.


The RA3-6B2 antibody is one of the most consistently used leukocyte markers for B cells, T cell subsets and NK cell subsets in human and mouse. This antibody has also been reported as cross-reactive with feline CD45R/B220.


Alternative Names
Ly-5, Lyt-4, T200
Gene ID
19264
Citations

Willinger T and Flavell RA. 2012. Proc. Natl. Acad. Sci. 109:8670-8675. (Flow cytometry)


Meredith MM, Liu K, Darrasse-Jeze G, Kamphorst AO, Schreiber HA, Guermonprez P, Idoyaga J, Cheong C, Yao K-H, Niec RE, and Nussenzweig MC. 2012. J. Exp. Med. 209: 1153-1165. (Immunofluorescence microscopy: acetone-fixed frozen tissue)


Becker-Herman A, Meyer-Bahlburg A, Schwartz MA, Jackson SW, Hudkins KL, Liu C, Sather BD, Khim S, Liggitt D, Song W, Silverman GJ, Alpers CE and Rawlings DJ. 2011. J. Exp. Med. 208:2033-2042. (Immunofluorescence microscopy - OCT embedded frozen tissue)


Bertossi A, Aichinger M, Sansonetti P, Lech , Neff F, Pal M, Wunderlich FT, Anders H, Klein L, and Schmidt-Supprian M. 2011. J. Exp. Med. 208:1749-1756. (Immunofluorescence microscopy)


De Clercq S, Gembarska A, Denecker G, Maetens M, Naessens M, Haigh K, Haigh JJ, and Marine J-C. 2010. Mol. Cell. Biol. 30:5394-5405. (Western Blot)


Nutt SL, Metcalf D, D´ Amico A, Polli M, and Wu L. 2005. J. Exp. Med. 201:221-231. (Immunomagnetic bead depletion)


Cappione AJ, Pugh-Bernard AE, Anolik JH, and Sanz I. 2004. J. Immunol. 172: 4298-4307. (Immunoprecipitation)


Monteith CE, Chelack BJ, Davis WC, and Haines DM. 1996. Can. J. Vet. Res. 60(3): 193-198. (Immunohistochemistry - feline tissue)


Whiteland JL, Nicholls SM, Shimeld C, Easty DL, Williams NA, and Hill TJ. 1995. J. Histochem. Cytochem. 43:313-320. (Immunohistochemistry - frozen tissue, paraffin embedded tissue)


Domiati-Saad R, Ogle EW, and Justement LB. 1993. J. Immunol. 151: 5936-5947. (in vivo blocking)


Cross Reactivity
Feline

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ug
Available: In stock
Listprice: €43.00
Price: €43.00
available

Delivery expected until 8/28/2025 

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