X-GAL

5-Bromo-4-chloro-3-indolyl β -D-galactopyranoside is most commonly known as  X-Gal. X-gal is a histochemical substrate for β -galactosidase which cleaves X-Gal to yield an insoluble blue precipitate.
X-Gal is a mainstay in molecular biology cloning applications in which it is used to detect the activity of β -galactosidase. X-Gal is used to detect the insertion of foreign DNA into the lacZ region of a plasmid DNA. Insertion of DNA into the lacZ region results in the loss of β -galactosidase activity.
X-gal is used to indicate whether a cell expresses the β -glactosidase enzyme, which is encoded by the lacZ gene, in a technique called blue white screening. Bacterial cells that retain active β -galactosidase will result in characteristic blue colonies. Successful disruption of the  lacZα   gene disrupts the α -complementation of the β -galactosidase gene and the precipitate does not form, resulting in white colonies.
 
 
 

5-Bromo-4-Chloro-3-Indolyl-B-D-Galactopyranoside

236-238 ° C

99% (TLC)

White Powder

X1009969-1

C₁₄H₁₅BrClNO₆

c1cc(c(c2c1[nH]cc2O[C@H]3[C@@H]([C@H]([C@H]([C@H](O3)CO)O)O)O)Cl)Br