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MERS-CoV (NCoV / Novel coronavirus) Spike Protein fragment (aa 383-502, Fc Tag)

MERS-CoV (NCoV / Novel coronavirus) Spike Protein fragment (aa 383-502, Fc Tag)

Item no.	40071-V31B-100
Manufacturer	Sino Biological
Amount	100ug
Category	Neuroscience Aging & Neurological Disorders Infectious Diseases Viral Infections Coronavirus (COVID-19) Peptides & Proteins Recombinant Proteins Flow Cytometry ELISA
Type	Proteins
Format	Baculovirus-Insect cells
Applications	FC, ELISA
Specific against	MERS-CoV
Host	Baculovirus-Infected Insect Cells
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	S
Similar products	Spike
Available
Molecule	Spike
Synonym	S
Storage	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction	A DNA sequence encoding the spike protein fragment (Human betacoronavirus 2c EMC/2012)(AFS88936.1)(Cys383-Lys502) was fused with the Fc region of rabbit IgG at the C-terminus.
Expressed Host	Baculovirus-Insect Cells
Purity	> 85 % as determined by SDS-PAGE
Bio-Activity	Measured by its binding ability in a functional ELISA. 1. Immobilized Spike (HCoV-EMC/2012) (Cat: 40071-V31B) at 10 ug/ml (100 ul/well) can bind biotinylated human ACE2-Fc (Cat: 10108-H02H). The EC50 of biotinylated human ACE2-Fc (Cat: 10108-H02H) is 0.04-0.06 ug/ml. 2. Immobilized Spike (HCoV-EMC/2012) (Cat: 40071-V31B) at 10 ug/ml (100 ul/well) can bind biotinylated DPP4 (Cat: 10688-HNCH). The EC50 of of biotinylated DPP4 (Cat: 10688-HNCH) is 0.10-0.16 ug/ml. 3. Immobilized Spike (HCoV-EMC/2012) (Cat: 40071-V31B) at 10 ug/ml (100 ul/well) can bind biotinylated Fc-DPP4 (Cat: 10688-H01H). The EC50 of biotinylated Fc-DPP4 (Cat: 10688-H01H) is 0.15-0.23 ug/ml.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Cys 383
Molecule Mass	The recombinant spike protein fragment (Human betacoronavirus 2c EMC/2012) comprises 344 amino acids and has a predicted molecular mass of 38.5 kDa. It migrates as an approximately 42 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 100mM Glycine, 100mM Nacl, pH 7.0.
Accession	AFS88936.1
Amino acid sequence	Cys383-Lys502
TAG	C-rabbit IgG-Fc

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100ug

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Delivery expected until 9/4/2025

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