VEGFR3/FLT4 Matched ELISA Antibody Pair Set,Human

Capture Antibody: Aliquot and store at -20C to -80C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.

Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20C to -80C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.

Standard: Store lyophilized standard at -20C to -80C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80C for up to 1 month. Avoid repeated freeze-thaw cycles.

0.5 mg/mL of mouse anti-VEGFR3 monoclonal antibody. Dilute to a working concentration of 2 ug/mL in CBS before coating

Each vial contains 80 ng of recombinant VEGFR3. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20C to -80C in a manual defrost freezer. A six-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 5 ng/mL is recommended

The human VEGFR3 / FLT4 ELISA Pair Set is for the quantitative determination of human VEGFR3 / FLT4.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for VEGFR3 /FLT4 coated on a 96-well plate. Standards and samples are added to the wells, and any VEGFR3 / FLT4 present binds to the immobilized antibody. The wells arewashed and a horseradish peroxidase conjugated mouse anti-VEGFR3 / FLT4monoclonal antibody is then added, producing an antibody-antigen-antibody"sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of VEGFR3 / FLT4 present inthe sample. To end the enzyme reaction, the stop solution is added andabsorbances of the microwell are read at 450 nm.