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Mouse Inflammation Array C1

Mouse Inflammation Array C1

€863.00

Excl. VAT

Item no.	AAM-INF-1-8
Manufacturer	Raybiotech
Amount	8 Sample Kit
Category	Oncology Neuroscience Neuroinflammation Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism Regulation of Appetite Neural Signaling By Cell Type Carcinoma Glioma Hematology Leukemia Lymphoma Cell Biology Cell Movement Cell Adhesion Obesity Cell Culture Cytokines Cell Status Necrosis Apoptosis Mesothelioma Endocrine Regulation of Lipid Metabolism Enzymes Fatty Acid Synthesis Arrays By Organ Testicular & Prostate Cancer Antibody Arrays
Type	Assay
Specific against	Mouse (Murine, Mus musculus)
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
Similar products	IFN-gamma, IL-10, IL-1 alpha, IL-1 beta, IL-6, TIMP-1, IL-13, IL-4, SDF-1, Fractalkine, G-CSF, IL-17, IL-2, RANTES, M-CSF, MIP-1 alpha, GM-CSF, IL-9, MCP-1, IL-3, Lymphotactin, MIG, TECK, TIMP-2, LIX, Eotaxin-2, I-TAC, IL-12 p70, Eotaxin-1, MIP-1 gamma, BLC, TNF alpha, Fas Ligand, CD30 Ligand, sTNFRI
Available
Short description	RayBio C-Series Mouse Inflammation Antibody Array 1 Kit. Detects 40 Mouse Inflammatory Factors. Suitable for all liquid sample types.
Features	Easy to use No specialized equipment needed Compatible with nearly any liquid sample Proven technology (many publications) Highly sensitive (pg/ml) Sandwich ELISA specificity Higher density than ELISA, Western blot or bead-based multiplex
Number of Targets Detected	40
Target Names	BLC (CXCL13), CD30 Ligand (TNFSF8), Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), Fas Ligand (TNFSF6), Fractalkine (CX3CL1), GCSF, GM-CSF, IFN-gamma, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-2, IL-3, IL-4, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17A, I-TAC (CXCL11), KC (CXCL1), Leptin, LIX, Lymphotactin (XCL1), MCP-1 (CCL2), M-CSF, MIG (CXCL9), MIP-1 alpha (CCL3), MIP-1 gamma, RANTES (CCL5), SDF-1 alpha (CXCL12 alpha), I-309 (TCA-3/CCL1), TECK (CCL25), TIMP-1, TIMP-2, TNF alpha, TNF RI (TNFRSF1A), TNF RII (TNFRSF1B)
Targets Detected	BLC, CCL1, CCL11, CCL2, CCL24, CCL25, CCL3, CCL5, CD30 Ligand, CX3CL1, CXCL1, CXCL11, CXCL12, CXCL13, CXCL9, Eotaxin-1, Eotaxin-2, Fas Ligand, Fractalkine, G-CSF, GM-CSF, I-309, I-TAC, IFN-gamma, IL-1 alpha, IL-1 beta, IL-1 F1, IL-1 F2, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, IL-2, IL-3, IL-4, IL-6, IL-9, KC, Leptin, LIX, Lymphotactin, M-CSF, MCP-1, MIG, MIP-1 alpha, MIP-1 gamma, MPIF-2, RANTES, SDF-1, TCA-3, TECK, TIMP-1, TIMP-2, TNF alpha, TNF RI, TNF RII, TNFSF6, TNFSF8, XCL1
Suggested Applications	Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker/ Key Factor Screening, Identifying Key Factors, Confirming a Biological Process
Kit Components	Mouse Inflammation Antibody Array C1 Membranes Blocking Buffer Wash Buffer 1 Wash Buffer 2 Biotinylated Detection Antibody Cocktail Streptavidin-Conjugated HRP Detection Buffer C Detection Buffer D Lysis Buffer 8-Well Incubation Tray Plastic Sheets Array Templates Manual
Other Materials Required	Pipettors, pipet tips and other common lab consumables Orbital shaker or oscillating rocker Tissue Paper, blotting paper or chromatography paper Adhesive tape or Saran Wrap Distilled or de-ionized water A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline	Block membranes Incubate with Sample Incubate with Biotinylated Detection Antibody Cocktail Incubate with HRP-Conjugated Streptavidin Incubate with Detection Buffers Image with chemiluminescent imaging system Perform densitometry and analysis
Storage	For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support	Membrane
Design Principle	Sandwich-based
Result Output	Semi-Quantitative
Detection Method	Chemiluminescence
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Citation	1. Wu Z., Chen X., Liu F., Chen W., Wu P., Wieschhaus A., Chishti A., Roche P., Chen W., Lin TJ. Calpain-1 Contributes to IgE-Mediated Mast Cell Activation. The Journal of Immunology April 23, 2014 1301677. doi: 10.4049/? [Epub ahead of print] 2. Ke, F., Zhang, L. et al. Autocrine IL-6 drives skin-derived mesenchymal stem cell trafficking via regulating voltage-gated Ca2+ channels. Stem Cells 2014. Epub Ahead of Print. DOI: 10.1002/stem.1763 3. Soki F., Polarization of Prostate Cancer Associated Macrophages is Induced by Milk-Fat Globule-EGF Factor 8 (MFG-E8) Mediated Efferocytosis. JBC Papers in Press. Published on July 8, 2014. http://www.jbc.org/cgi/doi/10.1074/jbc.M114.571620 4. Sun H., Adjuvant-active fraction from Albizia julibrissin saponins improves immune responses by inducing cytokine and chemokine at the site of injection. International Immunopharmacology 22 (2014) 346–355. http://dx.doi.org/10.1016/j.intimp.2014.07.021 5. Adkins I., et al. Bordetella Adenylate Cyclase Toxin Differentially Modulates Toll-Like Receptor-Stimulated Activation, Migration and T Cell Stimulatory Capacity of Dendritic Cells. PLOS One 2014. Published: August 01, 2014. DOI: 10.1371/journal.pone.0104064 6. Tsuyusaki J., et al. Cigarette smoke-induced pulmonary inflammation is attenuated in CD69-deficient mice. Journal of Receptors and Signal Transduction, 2011, 31(6): 434–439, DOI: 10.3109/10799893.2011.631929 7. Lesina E., et al. CpG-free plasmid DNA prevents deterioration of pulmonary function in mice. European Journal of Pharmaceutics and Biopharmaceutics 74 (2010) 427–434. doi:10.1016/j.ejpb.2009.11.013

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

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