Mouse Cytokine Array C2

• Easy to use
• No specialized equipment needed
• Compatible with nearly any liquid sample
• Proven technology (many publications)
• Highly sensitive (pg/ml)
• Sandwich ELISA specificity
• Higher density than ELISA, Western blot or bead-based multiplex

6Ckine (CCL21), CTACK (CCL27), Eotaxin-1 (CCL11), GCSF, GM-CSF, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17A, IFN-gamma, KC (CXCL1), Leptin, MCP-1 (CCL2), MCP-5, MIP-1 alpha (CCL3), MIP-2, MIP-3 beta (CCL19), RANTES (CCL5), SCF, TNF RI (TNFRSF1A), TARC (CCL17), TIMP-1, TNF alpha, Thrombopoietin (TPO), VEGF-A

Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker/ Key Factor Screening, Identifying Key Factors, Confirming a Biological Process

• Pipettors, pipet tips and other common lab consumables
• Orbital shaker or oscillating rocker
• Tissue Paper, blotting paper or chromatography paper
• Adhesive tape or Saran Wrap
• Distilled or de-ionized water
• A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

For best results, store the entire kit frozen a -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer a -20C and all other reagents undiluted at 4C for no more than 3 months.

Sandwich-based

Chemiluminescence

1. Zhu D., Can C., Purwanti Y., Du S., Lam D., Wu C., et al. Induced Pluripotent Stem Cell-derived Neural Stem Cells Transduced with Baculovirus Encoding CD40 Ligand for Immunogene Therapy in Mouse Models of Breast Cancer. Human Gene Therapy Online Ahead of Editing: April 28th, 2014. doi:10.1089/hum.2013.160. [Epub ahead of print]
2. Honko AN, Mizel SB. Mucosal Administration of Flagellin Induces Innate Immunity in the Mouse Lung. Infect Immun. 2004, 72(11):6676-6679.
3. Rich J, Lee J. The Pathogenesis of Staphylococcus aureus Infection in the Diabetic NOD Mouse. Diabetes. 2005, 54:2904–2910.
4. Wu, Q et al. Monitoring the response of orthotopic bladder tumors to granulocyte macrophage colony-stimulating factor therapy using the prostate-specific antigen gene as a reporter. Monitoring the response of orthotopic bladder tumors to granulocyte macrophage colony-stimulating factor therapy using the prostate-specific antigen gene as a reporter. Monitoring the response of orthotopic bladder tumors to granulocyte macrophage colony-stimulating factor therapy using the prostate-specific antigen gene as a reporter. Clin. Cancer Res. Volume 10, Issue 20, Pages 6977-84, Oct 2004
5. Fan, H et al. Cytokine dysregulation induced by apoptotic cells is a shared characteristic of macrophages from nonobese diabetic and systemic lupus erythematosus-prone mice. J. Immunol. Volume 172, Issue 8, Pages 4834-43, Apr 2004
6. Michel, M., et al. Identification of an IL-17–producing NK1.1neg iNKT cell population involved in airway neutrophilia. Journal of experimental Medicine. 2007, vol. 204 no. 5 995-1001. doi: 10.1084/jem.20061551
7. Jung Y., Kim M., Soh H., Lee S., et al. TC1(C8orf4) Regulates Hematopoietic Stem/Progenitor Cells and Hematopoiesis. PLOS One. Published: June 17, 2014 DOI: 10.1371/journal.pone.0100311
8. Herrmann, A., Priceman, S., et al. CTLA4 aptamer delivers STAT3 siRNA to tumor-associated and malignant T cells. The Journal of Clinical Investigation 2014. Epub Ahead of Print DOI: 10.1172/JCI73174
9. Ehninger D. Tsc2 Haploinsufficiency Has Limited Effects on Fetal Brain Cytokine Levels during Gestational Immune Activation. Autism Research and Treatment. Volume 2014, Article ID 761279, 7 pages http://dx.doi.org/10.1155/2014/761279
10. Shan Y., er al. Suppression of mucin 2 promotes interleukin-6 secretion and tumor growth in an orthotopic immune-competent colon cancer animal model. ONCOLOGY REPORTS 32: 2335-2342, 2014, DOI: 10.3892/or.2014.3544