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Human Angiogenesis Array Q2

Human Angiogenesis Array Q2

Item no.	QAH-ANG-2-1
Manufacturer	Raybiotech
Amount	8 Sample Kit
Category	Oncology Neuroscience Neural Lineage Markers Neuroinflammation Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism Regulation of Appetite Neural Signaling By Cell Type Carcinoma Hematology Leukemia Cell Biology Cell Movement Cell Adhesion Obesity Cell Culture Cytokines Cell Status Necrosis Mesothelioma Endocrine Regulation of Lipid Metabolism Arrays By Organ Bladder Cancer Colorectal Cancer Testicular & Prostate Cancer Antibody Arrays
Type	Assay
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
Available
Short description	Quantibody Human Angiogenesis Array 2 Kit. Detects 30 Human Angiogenic Factors. Suitable for all liquid sample types.
Features	More cost-effective than traditional ELISA High specificity and system reproducibility Suitable for diverse sample types Low sample volume requirement: 50 uL or less Get results same day (6-hour processing time) Well-suited for high throughput assays Q Analyzer software provides one-step computation
Number of Targets Detected	30
Target Names	Activin A, AgRP, Angiogenin, Angiopoietin-2, ANGPTL4, bFGF, ENA-78 (CXCL5), GRO alpha/beta/gamma, HB-EGF, HGF, IFN-gamma, IGF-1, IL-1 alpha (IL-1 F1), IL-2, IL-6, IL-8 (CXCL8), IL-17A, IP-10 (CXCL10), Leptin, LIF, MCP-1 (CCL2), PDGF-BB, PLGF, RANTES (CCL5), TGF beta 1, TIMP-1, TIMP-2
Targets Detected	Activin A, AgRP, Angiogenin, Angiopoietin-2, ANGPTL4, bFGF, CCL2, CCL5, CXCL10, CXCL5, CXCL8, ENA-78, GRO, HB-EGF, HGF, IFN-gamma, IGF-1, IL-1 alpha, IL-1 F1, IL-17, IL-2, IL-6, IL-8, IP-10, Leptin, LIF, MCP-1, PDGF-BB, PlGF, RANTES, TGF beta 1, TIMP-1, TIMP-2
Suggested Applications	Multiplexed Protein Detection, Biomarker Screening, Identifying Key Factors, Confirming Biological Process, Biomarker Validation, Validation of Antibody Array Results, Quantitative Protein Detection, Establishing Normal Range
Kit Components	Human Angiogenesis Array Q2 Slide(s) Blocking Buffer Wash Buffer 1 Wash Buffer 2 Lyophilized Standard Mix Biotinylated Detection Antibody Cocktail Streptavidin-Conjugated Fluor 30 ml Centrifuge Tube Adhesive Plastic Strips Manual
Other Materials Required	Distilled or deionized water Small plastic boxes or containers Pipettors, pipette tips and other common lab consumables Orbital shaker or oscillating rocker Aluminum foil Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline	Dry the glass slide Prepare Standards Block array surface Incubate with Samples and Standards Incubate with Biotinylated Detection Antibody Cocktail Incubate with Streptavidin-Conjugated Fluor Disassemble the glass slide Scan with a gene microarray laser scanner Perform densitometry and analysis
Storage	For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support	Glass Slide
Design Principle	Sandwich-based
Result Output	Quantitative
Detection Method	Fluorescence Laser Scanner
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Citation	1. Das K., et al. Differential expression of steroid 5alpha-reductase isozymes and association with disease severity and angiogenic genes predict their biological role in prostate cancer. Endocr Relat Cancer. 2010 Aug 16, 17(3):757-70. doi: 10.1677/ERC-10-0022.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.