Biotinylated HCoV-HKU1 (isolate N5) Spike Trimer, His,Avitag™ (MALS verified)

PBS

Human coronavirus HKU1 (HCoV-HKU1) is one of seven known coronaviruses to infect humans. The virus is an enveloped, positive-sense, single-stranded RNA virus which enters its host cell by binding to the N-acetyl-9-O-acetylneuraminic acid receptor. It causes an upper respiratory disease with symptoms of the common cold, but can advance to pneumonia and bronchiolitis. The spike protein of HCoV-HKU1 is a trimer that contains two main subunits: the S1 subunit is responsible for receptor binding, while the S2 subunit mediates membrane fusion.

Ala 13 - Asn 1276

147.2 kDa

Biotinylated HCoV-HKU1 (isolate N5) Spike Trimer, His, Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Biotinylated HCoV-HKU1 (isolate N5) Spike Trimer, His, Avitag (SPN-H82E3) is expressed from human 293 cells (HEK293). It contains AA Ala 13 - Asn 1276 (Accession # Q0ZME7-1). Amino acid substitutions RRKRR 752-756 GGSGS and NL 1067-1068 PP are introduced to abolish the furin cleavage site and stabilize the trimeric prefusion state of the spike protein, respectively.