HCoV-HKU1 (isolate N5) Spike Trimer, His Tag (MALS verified)

PBS

Human coronavirus HKU1 (HCoV-HKU1) is one of seven known coronaviruses to infect humans. The virus is an enveloped, positive-sense, single-stranded RNA virus which enters its host cell by binding to the N-acetyl-9-O-acetylneuraminic acid receptor. It causes an upper respiratory disease with symptoms of the common cold, but can advance to pneumonia and bronchiolitis. The spike protein of HCoV-HKU1 is a trimer that contains two main subunits: the S1 subunit is responsible for receptor binding, while the S2 subunit mediates membrane fusion.

Ala 13 - Asn 1276

145.5 kDa

HCoV-HKU1 (isolate N5) Spike Trimer, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

HCoV-HKU1 (isolate N5) Spike Trimer, His Tag (SPN-H52H5) is the ectodomain of HCoV-HKU1 (isolate N5) spike protein which contains AA Ala 13 - Asn 1276 (Accession # Q0ZME7-1). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. The S1/S2 furin-recognition site 752-RRKRR-756 is mutated to GGSGS to generate the uncleaved protein. Proline substitutions (N1067P, L1068P) are introduced to stabilize the trimeric prefusion state of the spike protein.