SARS-CoV-2 Spike Trimer (G75V, T76I, SYLTPGD 247-253 del, L452Q, F490S, D614G, T859N), His Tag (MALS verified)

Immobilized SARS-CoV-2 Spike Trimer, His Tag (Cat. No. SPN-C52Hs) at 1 ug/mL (100 uL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.1-6 ng/mL (QC tested).

PBS

It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Val 16 - Pro 1213

137.3 kDa

SARS-CoV-2 Spike Trimer, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SARS-CoV-2 Spike Trimer, His Tag (SPN-C52Hs) is the ectodomain of SARS-CoV-2 spike protein which contains AA Val 16 - Pro 1213 (Accession # QHD43416.1). Mutations G75V, T76I, SYLTPGD 247-253 del, L452Q, F490S, D614G, T859N were identified in the SARS-CoV-2 Lambda variant (Pango lineage: C.37). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.