SARS-CoV-2 S protein RBD (N501Y), Fc Tag (MALS verified)

Immobilized SARS-CoV-2 S protein RBD (N501Y), Fc Tag (Cat. No. SPD-C5253) at 2 ?g/mL (100 ?L/well) can bind Human ACE2, His Tag (Cat. No. AC2-H52H8) with a linear range of 39-156 ng/mL (QC tested).

50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5

It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Arg 319 - Lys 537

51.1 kDa

SARS-CoV-2 S protein RBD (N501Y), Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.