Recombinant Human FGF2 Protein

No tag

< 1.0 EU/μg of the protein by LAL method.

Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Growth Factor, Cell Culture related

Lyophilized from a 0.22 μm filtered solution of 20mM Tris，150 mM NaCl, pH7.5.Contact us for customized product form or formulation.

1.Measured by its binding ability in a functional ELISA. Immobilized Human FGF2 at 0.5 μg/mL (100 μL/well) can bind Human GPC3 with a linear range of 7-20 ng/mL.|2.Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts. The ED₅₀ for this effect is typically 0.635-2.54 ng/mL, corresponding to a specific activity of 3.94 × 10⁵~1.57 × 10⁶ units/mg.|3.Recombinant Human VEGFA(40 ng/mL, Cat. RP01162) and bFGF(50 ng/mL) induce mesoderm cells to differentiate into hematopoietic stem and progenitor cells. After 4 days induction, pebbly-like CD43+ hematopoietic stem and progenitor cells appeared in the hematogenic endothelium.|4.The primary neural stem cells were cultured with 20 ng/mL bFGF and observed every 24 h. Results showed that the particle size of the suspended neural stem cells gradually increased.