Recombinant mouse IL-3R alpha/CD123 protein

SDLAAVREAP PTAVTTPIQN LHIDPAHYTL SWDPAPGADI TTGAFCRKGR DIFVWADPGL ARCSFQSLSL CHVTNFTVFL GKDRAVAGSI QFPPDDDGDH EAAAQDLRCW VHEGQLSCQW ERGPKATGDV HYRMFWRDVR LGPAHNRECP HYHSLDVNTA GPAPHGGHEG CTLDLDTVLG STPNSPDLVP QVTITVNGSG RAGPVPCMDN TVDLQRAEVL APPTLTVECN GSEAHARWVA RNRFHHGLLG YTLQVNQSSR SEPQEYNVSI PHFWVPNAGA ISFRVKSRSE VYPRKLSSWS EAWGLVCPPE VMPVK

IL-3R alpha/CD123, also known as interleukin 3 receptor subunit alpha, is a member of the type 1 cytokine receptor family and type 5 subfamily. It is expressed on multiple cell types, including endothelial cells, monocytes, eosinophils, basophils plus mast cells, and plasmacytoid CD4+ T cells. It serves as a low affinity binding protein for monomeric IL‑3. The IL‑3 and IL‑3 R alpha complex likely interacts with a preformed signaling homodimer comprised of either beta IL-3 or beta c chains. The extracellular domain of mouse IL‑3 R alpha/CD123 shares only 44% and 29% aa sequence identity with rat and human IL‑3 R alpha, respectively. Nevertheless, human IL‑3 is reported to be active on the mouse receptor complex that is expressed on select cell types. Also, the specific alpha subunit of the interleukin 3 receptor is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias. Recombinant mouse IL-3R alpha/CD123, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

< 1 EU per 1ug of protein (determined by LAL method)

61.5kDa (554aa)

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.