Comparison

Recombinant human FAP protein

€558.00
Excl. VAT
Item no. NKM-ATGP3988-50ug
Manufacturer NKMAX
Amount 50ug
Category
Type Proteins Recombinant
Applications SDS-PAGE
Specific against other
Conjugate/Tag His-Tag
Purity > 90% by SDS-PAGE
NCBI NP_004451.2
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias Fibroblast activation protein, DPPIV Protein, DPPIV, FAPA, Fapalpha Protein, SIMP Protein, Prolyl endopeptidase FAP, 170 kDa melanoma membrane-bound gelatinase, Dipeptidyl peptidase FAP, Fibroblast activation protein alpha, FAPalpha, Gelatine degradation protease FAP, Integral membrane serine protease, Post-proline cleaving enzyme, Serine integral membrane protease, SIMP, Surface-expressed protease, Seprase
Available
Antigen Species
Human
Bioactivity Comment Only
Specific activity is > 5, 000 pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1.0 pmole of Z-GP-AMC per minute at pH 7.5, at 37C.
Bioactivity Comment and Pics
Specific activity is > 5, 000 pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1.0 pmole of Z-GP-AMC per minute at pH 7.5, at 37C.
Bioassay Procedure
LRPSRVH NSEENTMRAL TLKDILNGTF SYKTFFPNWI SGQEYLHQSA DNNIVLYNIE TGQSYTILSN RTMKSVNASN YGLSPDRQFV YLESDYSKLW RYSYTATYYI YDLSNGEFVR GNELPRPIQY LCWSPVGSKL AYVYQNNIYL KQRPGDPPFQ ITFNGRENKI FNGIPDWVYE EEMLATKYAL WWSPNGKFLA YAEFNDTDIP VIAYSYYGDE QYPRTINIPY PKAGAKNPVV RIFIIDTTYP AYVGPQEVPV PAMIASSDYY FSWLTWVTDE RVCLQWLKRV QNVSVLSICD FREDWQTWDC PKTQEHIEES RTGWAGGFFV STPVFSYDAI SYYKIFSDKD GYKHIHYIKD TVENAIQITS GKWEAINIFR VTQDSLFYSS NEFEEYPGRR NIYRISIGSY PPSKKCVTCH LRKERCQYYT ASFSDYAKYY ALVCYGPGIP ISTLHDGRTD QEIKILEENK ELENALKNIQ LPKEEIKKLE VDEITLWYKM ILPPQFDRSK KYPLLIQVYG GPCSQSVRSV FAVNWISYLA SKEGMVIALV DGRGTAFQGD KLLYAVYRKL GVYEVEDQIT AVRKFIEMGF IDEKRIAIWG WSYGGYVSSL ALASGTGLFK CGIAVAPVSS WEYYASVYTE RFMGLPTKDD NLEHYKNSTV MARAEYFRNV DYLLIHGTAD DNVHFQNSAQ IAKALVNAQV DFQAMWYSDQ NHGLSGLSTN HLYTHMTHFL KQCFSLSD
Concentration
0.25mg/ml (determined by absorbance at 280nm)
Description
FAP, also known as Seprase, is a homodimeric integral membrane gelatinase belonging to the serine protease family. FAP includes dipeptidyl peptidase IV (DPPIV / CD26) and related type II transmembrane prolyl serine peptidases, which exert their mechanisms of action on the cell surface. And it's enzymatic activity is dependent on FAP association with DPPIV on the cell surface. The active site of FAP is localized in the extracellular part of the protein and contains a catalytic triad composed of Ser624 Asp702 His734 in humans and mice. It is catalytically active as a 170kD homodimer and has a dipeptidase and an endopeptidase activity. FAP expression is high in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. FAP is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. ecombinant human FAP, fused to His-tag at C-terminus, was expressed in insert cell and purified by using conventional chromatography techniques.
Domain
26-760aa
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Expression System
Baculovirus
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol
Gene Category
Cancer, Enzymes & Proteases, Cell Cycle
General References
Scanlan, M.J. et al. (1994) Proc. Natl. Acad. Sci. USA 91:5657-5661.; ; Mori, Y. et al., 2004, Oncology. 67 (5-6):411-419.; ; O'Brien, P. et al., 2008, Biochim Biophys Acta. 1784 (9):1130-1145.
Molecular Weight
86.1 kDa (744aa)
Predicted nterm
Ala

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 50ug
Available: In stock
Listprice: €558.00
Price: €558.00
available

Delivery expected until 8/28/2025 

Compare

Add to wishlist

Get an offer

Request delivery time

Ask a technical question

Submit a bulk request

Questions about this Product?
 
Close