Recombinant human CTLA-4 protein

Human

The activity is determined by the IL-2 ELISA in a using Jurkat human acute T cell leukemia cells. The ED50 range ??150 ng/ml with Human B7-1/CD80.

CTLA-4, also known as cytotoxic T-lymphocyte protein 4, is a member of the immunoglobulin superfamily that is expressed by activated T cells and transmits an inhibitory signal to T cells. It also is a protein receptor that functions as an immune checkpoint and downregulates immune responses. This protein is constitutively expressed in regulatory T cells but only upregulated in conventional T cells after activation a phenomenon which is particularly notable in cancers. It may also function via modulation of cell motility and signaling through PI3 kinase. It has been implicated in several autoimmune diseases, such as systemic lupus erythematosus. Recombinant human CTLA-4, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

< 1 EU per 1ug of protein (determined by LAL method)

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol

Dai F., et al. (2015) Braz J Med Biol Res. 48:629-636.
Ward FJ., et al. (2013) Eur J Immunol. 43:1274-1285.

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.