Recombinant human ICOS protein

ADPEINGSAN YEMFIFHNGG VQILCKYPDI VQQFKMQLLK GGQILCDLTK TKGSGNTVSI KSLKFCHSQL SNNSVSFFLY NLDHSHANYY FCNLSIFDPP PFKVTLTGGY LHIYESQLCC QLKLEPKSCD KTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSRDELTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGKHHHH HH

ICOS, also known as inducible T-cell costimulator, is a member of the CD28 family of immune-assisted stimulatory receptors. The interaction of B7-H2 / ICOS plays a crucial role in Th cell differentiation, T-B cell interaction and humoral immune response essential for the formation of reproductive centers and the production of cytokine IL-4. In addition, this protein is more potent in inducing IL-10 production, a cytokine important for the inhibitory function of T regulatory cells. The B7-1 / B7-2-CD28 / CTLA-4 and ICOS-B7RP-1 pathways provide an important second signal that can regulate the activation, inhibition and fine regulation of T-lymphocyte responses. It stimulates production of Th1 and Th2 cytokines, but may play a role in the generation of Th2 cells. Recombinant human ICOS, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

< 1 EU per 1ug of protein (determined by LAL method)

Liquid in. 20mM MES buffer (pH 5.5) containing 40% glycerol, 2mM DTT, 1mM EDTA 0.1M NaCl

Gonzalo, J.A., et al. (2001) J. Immunol. 166:1-5.; ; Tezukak., et al. (2000) Biochem Biophys Res Commun. 276:335-345

Ala