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Recombinant rat TNF-alpha protein

Recombinant rat TNF-alpha protein

€137.00

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Item no.	NKM-ATGP3736-10ug
Manufacturer	NKMAX
Amount	10ug
Category	Oncology Neuroscience Neuroinflammation Metabolism Diabetes By Cell Type Peptides & Proteins Recombinant Proteins Cell Biology Obesity Cell Culture Cytokines Cell Status Necrosis Apoptosis SDS-PAGE
Type	Proteins Recombinant
Applications	SDS-PAGE
Specific against	other
Conjugate/Tag	His-Tag
Purity	> 90% by SDS-PAGE
NCBI	NP_036807
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Tumor necrosis factor, RATTNF, TNF-alpha, Tnfa
Available
Antigen Species	Rat
Bioactivity Comment Only	Measured in a cytotoxicity assay using L929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range <=0.2ng/ml.
Bioactivity Comment and Pics	Rat TNF-alpha stimulates cytotoxicity of the L929 mouse fibroblast cells. The ED50 range <=0.2 ng/ml.
Bioassay Procedure	LRSSS QNSSDKPVAH VVANHQAEEQ LEWLSQRANA LLANGMDLKD NQLVVPADGL YLIYSQVLFK GQGCPDYVLL THTVSRFAIS YQEKVSLLSA IKSPCPKDTP EGAELKPWYE PMYLGGVFQL EKGDLLSAEV NLPKYLDITE SGQVYFGVIA L
Concentration	1mg/ml (determined by absorbance at 280nm)
Description	Tnf also known as Tumor necrosis factor. The Protein acts as a cytokine binds TNF receptors plays a role in regulation of cell proliferation, induction of apoptosis, and inflammatory response. Recombinant rat Tnf, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Domain	80-235aa
Endotoxin	< 1 EU per 1ug of protein (determined by LAL method)
Expression System	E.coli
Formulation	Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT
Gene Category	Hormones & Cytokines
General References	Furuya T., et al. (2001) Genes Immun. 2:229-232.; ; Warle M.C., et al. (2005) Transpl. Immunol. 14:77-82.
Molecular Weight	19.9 kDa (181aa) confirmed by MALDI-TOF

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 10ug

Listprice: €137.00

Price: €137.00

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Delivery expected until 8/28/2025

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