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Recombinant e.coli Beta-lactamase protein

Recombinant e.coli Beta-lactamase protein

€670.00

Excl. VAT

Item no.	NKM-ATGP3575-250ug
Manufacturer	NKMAX
Amount	250ug
Category	Food Safety Antibiotics Peptides & Proteins Recombinant Proteins SDS-PAGE
Type	Proteins Recombinant
Applications	SDS-PAGE
Specific against	other
Conjugate/Tag	His-Tag
Purity	> 95% by SDS-PAGE
NCBI	NP_418574
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	ampC, ampA, Cephalosporinase
Available
Antigen Species	E.coli
Bioactivity Comment Only	Specific activity is >700unit/mg, in which One unit will hydrolyze 1.0umole of Nitrocefin per minute at pH 7.0 at 37C
Bioactivity Comment and Pics	Specific activity is >700 units/mg, in which One unit will hydrolyze 1.0umole of Nitrocefin per minute at pH 7.0 at 37C
Bioassay Procedure	APQQINDIV HRTITPLIEQ QKIPGMAVAV IYQGKPYYFT WGYADIAKKQ PVTQQTLFEL GSVSKTFTGV LGGDAIARGE IKLSDPTTKY WPELTAKQWN GITLLHLATY TAGGLPLQVP DEVKSSSDLL RFYQNWQPAW APGTQRLYAN SSIGLFGALA VKPSGLSFEQ AMQTRVFQPL KLNHTWINVP PAEEKNYAWG YREGKAVHVS PGALDAEAYG VKSTIEDMAR WVQSNLKPLD INEKTLQQGI QLAQSRYWQT GDMYQGLGWE MLDWPVNPDS IINGSDNKIA LAARPVKAIT PPTPAVRASW VHKTGATGGF GSYVAFIPEK ELGIVMLANK NYPNPARVDA AWQILNALQ
Concentration	1mg/ml (determined by Bradford assay)
Description	AmpC, also known as Beta-lactamase, is the most widespread resistance mechanism to beta-lactam antibiotics, such as the penicillins and the cephalosporins. These antibiotics have a common element in their molecular structure: a four-atom ring known as a beta-lactam. The lactamase enzyme breaks that ring open, deactivating the molecule's antibacterial properties. Recombinant E. coli beta-lactamase protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Domain	20-377aa
Expression System	E.coli
Formulation	Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Gene Category	Metabolism
General References	Zhao Z., et al. (2015) Cancer Lett. 358(1):17-26.; ; Martin I., et al. (2014) Cell. 157(2):472-85.
Molecular Weight	41.8 kDa (379aa) confirmed by MALDI-TOF

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 250ug

Listprice: €670.00

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Delivery expected until 8/28/2025

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