Recombinant human CTLA-4 protein

Human

CTLA4 is a member of the immunoglobulin superfamily and transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. Recombinant human CTLA4 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Rudd CE, Taylor A, et al. (2009). Immunol Rev. 229(1):12-26.; ; Stamper CC, Zhang Y, et al. (2001). Nature. 410(6828):608-11.

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.