Recombinant Human Angiotensin-converting enzyme 2(ACE2),partial (Active)

ACE2

Mammalian cell

18-740aa

QSTIEEQAKTFLDKFNHEAEDLFYQSSLAS WNYNTNITEENVQNMNNAGDKWSAFLK EQSTLAQMYPLQEIQNLTVKLQLQALQQNGSS VLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQ ECLLLEPGLNEIMANSLDYNERLWAWES WRSEVGKQLRPLYEEYVVLKNEMARANHYED YGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLY EHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTN LYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSV GLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDD FLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLK SIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVS

Greater than 85% as determined by SDS-PAGE.

Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S (CSB-MP3324GMY) at 2 ug/ml can bind human ACE2, the EC50 of human ACE2 protein is 56.64 - 103.6 ng/ml. Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD (CSB-YP3324GMY1) at 5 ug/ml can bind human ACE2, the EC50 of human ACE2 protein is 31.80 - 44.69 ng/ml. Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD (CSB-MP3324GMY1b1) at 5 ug/ml can bind human ACE2, the EC50 of human ACE2 protein is 115.0 - 274.9 ng/ml.

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference.