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Human CD3 epsilon&CD3 gamma Heterodimer Protein, His Tag&Tag Free (MALS verified) European Partner

€3,185.00
Excl. VAT
Item no. CDG-H52W5-1mg
Manufacturer ACROBiosystems
Amount 1mg
Category
Type Proteins Recombinant
Format Powder
Specific against Human (Homo sapiens)
Conjugate/Tag His
Purity >90% as determined by SDS-PAGE.
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Available
Bioactivity Legend 1
Immobilized Human CD3E&CD3G Heterodimer Protein, His Tag&Tag Free (Cat. No. CDG-H52W5) at 1 ?g/mL (100 ?L/well) can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3) with a linear range of 0.1-3 ng/mL (QC tested).
Bioactivity Legend 2
Immobilized Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3) at 5 ?g/mL (100 ?L/well) can bind Human CD3E&CD3G Heterodimer Protein, His Tag&Tag Free (Cat. No. CDG-H52W5) with a linear range of 0.039-0.156 ?g/mL (Routinely tested).
Buffer
PBS, pH7.4
Characteristics
Human CD3E&CD3G Heterodimer Protein, His Tag&Tag Free is produced by co-expression of CD3E and CD3G, has a calculated MW of 16.9 kDa (CD3E) and 14.6 kDa (CD3G). Subunit CD3E is fused with a polyhistidine tag at the C-terminus and subunit CD3G contains no tag at the C-terminus. The reducing (R) heterodimer protein migrates as 19 kDa and 21-28 kDa due to glycosylation.
Description
T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
Endotoxin Level
Less than 1.0 EU per ?g by the LAL method.
Exp Region
Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G)
Expression System
HEK293
Molecular Weight
16.9 kDa | 14.6 kDa
Molecule
CD3E & CD3G
SDS-PAGE Legend
Human CD3E&CD3G Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Storage
-20?

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 1mg
Available: In stock
Listprice: €3,185.00
Price: €3,185.00
available

Delivery expected until 9/18/2025 

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