Human Erythropoietin (EPO) Protein (Active)

Erythropoietin Protein (Recombinant) is an active Human protein. It is produced in 293F cells using Eukaryotic expression.

Recombinant

> 92%

Calculated MW: 20.0 kDa
Observed MW: 33 kDa, 40 kDa (determined by SDS-PAGE)

Possible reasons why the actual band size differs from the predicted band size:
1. Splice variants. Alternative splicing may create different sized proteins from the same gene.
2. Relative charge. The composition of amino acids may affect the charge of the protein.
3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size.
4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form.
5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed.

No