EasyScript® All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal)

5×EasyScript® All-in-One SuperMix for qPCR provides all the necessary reagents for cDNA synthesis from total RNA or mRNA (including 5×EasyScript® RT, RNase Inhibitor, Anchored Oligo (dT)18 Primer, Random Primer (N9), dNTPs, Buffer). It is provided at 5× concentration and used at 1× concentration by only adding gDNA remover, RNA template and H2O for efficient first-strand cDNA synthesis. Simultaneously, residual genomic DNA from RNA template can be removed. 5×EasyScript® All-in-One No-RT Control SuperMix for qPCR is supplied to prepare no-reverse transcriptase (RT) control, which is used to assess if the qPCR template is contaminated with genomic DNA. This product is capable of minimizing contamination during operation with a simple workflow. The resulting cDNA is only suitable for qPCR, not for regular PCR.

High-copy-number and low-copy number gene detection