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MCF-7 Lysate

MCF-7 Lysate

€202.00

Excl. VAT

Item no.	NKM-ATGL0009-200ug
Manufacturer	NKMAX
Amount	200ug
Category	Western Blot Cell Lines & Cell Culture Cell Lysates
Type	Cell Lysate
Applications	WB
Specific against	other
ECLASS 10.1	32160890
ECLASS 11.0	32160890
UNSPSC	41105330
Available
Antigen Species	Human
Concentration	2mg/ml (determined by BCA assay)
Description	MCF-7 cells are breast cancer cell line isolated in 1970 from a 69 year old woman, in 1970s. MCF-7 cells are a well characterized estrogen receptor (ER) positive control cell line (cells are positive for cytoplasmic estrogen receptors) and therefore are a useful in vitro model of breast cancer to study the role of estrogen in breast cancer. Cells are also positive for cytokeratin and negative for desmin, endothelin, GFAP, neurofilament, vimentin.
Formulation	Liquid. In SDS-PAGE Sample Buffer (0.12M Tris-HCl pH6.8, 0.4% SDS, 5% Glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
Lysate QC	Western Blot analyzed by both GAPDH and Beta tubulin antibody
Preparation before usage	Heat lysate to 95C for 5 minutes and rapidly cooling before use.
Storage	Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 200ug

Listprice: €202.00

Price: €202.00

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Delivery expected until 8/28/2025

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