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293T Lysate

293T Lysate

€173.00

Excl. VAT

Item no.	NKM-ATGL0002-100ug
Manufacturer	NKMAX
Amount	100ug
Category	Western Blot Cell Lines & Cell Culture Cell Lysates
Type	Cell Lysate
Applications	WB
Specific against	other
ECLASS 10.1	32160890
ECLASS 11.0	32160890
UNSPSC	41105330
Available
Antigen Species	Human
Concentration	2mg/ml (determined by BCA assay)
Description	293T cells are a human embryonic kidney (HEK) cell line that expresses the large T antigen. The large T antigen is commonly used for transfection assays and is required for transient transfection of plasmids with an SV40 origin of replication. 293T cells are able to grow well in reduced serum medium or in medium lacking calcium ions. In addition, 293T cells tend to grow at a faster rate and to a higher cell density than the parent 293 cell line, resulting in increased amounts of endogenously expressed protein.
Formulation	Liquid. In SDS-PAGE Sample Buffer (0.12M Tris-HCl pH6.8, 0.4% SDS, 5% Glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
Lysate QC	Western Blot analyzed by both GAPDH and Beta tubulin antibody
Preparation before usage	Heat lysate to 95C for 5 minutes and rapidly cooling before use.
Storage	Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100ug

Listprice: €173.00

Price: €173.00

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Delivery expected until 8/28/2025

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