Human Medulla Oblongata Tissue Lysate

Details

Tissue Type

Brain: Medulla Oblongata

Diagnosis

Normal

Preparation

Human medulla oblongata tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70˚C. The human medulla oblongata tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the medulla oblongata tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The medulla oblongata tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Safety Testing

This material has been tested by accepted techniques and has been found to be negative for HBsAg, HIV 1/2, and HCV. Additional patient information may be available upon request.

Host

Human

Concentration

3 mg/ml

Formulation

The human medulla oblongata tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors.

Expiration Date

One half year from date of receipt

Storage

This lysate should be aliquoted in working amounts and may be stored for up to one month at 2-8˚C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. For longer term storage, freeze working aliquots at -70°C.

Applications and Suggested Working Dilutions

This lysate is for use in Western blotting, 10 µg to 20 µg per lane is recommended for mini gel.