Human Lymph Node Normal Tissue Lysate

Details

Tissue Type

Lymph Node

Diagnosis

Normal

Preparation

Lymph Node tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation.

Safety Testing

This material has been tested by accepted techniques and has been found to be negative for HBsAg, HIV 1/2, and HCV. Additional patient information may be available upon request.

Host

Human

Concentration

2.0 mg/ml

Formulation

The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% ß-mercaptoethanol.

Expiration Date

One year from date of receipt

Storage

For short term storage of this tissue lysate, freeze in single use aliquots at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. For long term storage freeze working aliquots at -70°C.

Applications and Suggested Working Dilutions

This lysate is for use in Western blotting, 10 µg to 20 µg per lane is recommended for mini gel.