Contents

• Introduction
• Key Principles of Molecular Biology Techniques
1. Molecular Cloning Basics (Library Construction)
1. cDNA Libraries
2. Genomic Libraries
2. Polymerase Chain Reaction (PCR)
1. PCR Fundamental Principles
2. Data Acquisition and Analysis: Gel Electrophoresis
3. qRT-PCR Experimental Basics
4. Data Acquisition and Analysis: Basic Calculations
• Sample Preparation and Protocols
1. Extraction & Purification Protocols
1. DNA Extraction & Purification
2. RNA Extraction & Purification
2. Molecular Cloning: DNA Library Preparation
3. PCR Protocols
1. Primer Design
2. General PCR protocol
3. PCR Product Analysis: Gel Electrophoresis
4. Reverse transcription protocol
5. qRT-PCR
• Troubleshooting Guide
1. DNA and RNA extraction
2. PCR and qRT-PCR
• FAQs
• Appendix: Fundamentals of Molecular Biology