CD57

GWB-A7D915

27087

TB01

Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3. X63 Ag8. 653 myeloma cell line.

This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections but sodium citrate buffer may enhance staining. Histology: Human Tonsil

Phosphate buffered saline pH7. 4

Use 10ul of the suggested working dilution to label 106 cells in 100ul. Suggested

Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR) asialo-fetuin and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid regardless of the length of the acyl group (By similarity).

Protein modification; protein glycosylation.

Golgi apparatus membrane; Single-pass type II membrane protein.

Belongs to the glycosyltransferase 43 family. 1. Funaro. A. et al. (1995) Epitope analysis of human CD57 by means of a panel of newly-generated high-affinity murine monoclonal antibodies. In Leucocyte Typing V: White Cell Differentiation Antigens. 2. Funaro. A. et al. (1995) Human CD57. a link molecule between leucocyte and neural cells. In: Leucocyte Typing V: White Cell Differentiation Antigens.