Vimentin

Purified human Vimentin

Sarcomas melanomas Hela cells

Cytoplasmic

10 mM PO4 150 mM NaCl pH 7. 4 0. 05% sodium azide.

Cytoplasmic Vimentin is the major subunit protein of the intermediate filaments of mesenchumal cells and has a molecular weight of 57 kD. Immunohistochemical staining for Vimentin is thus characteristic of sarcomas (of neural muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of malignant tumors generally used with a panel of other antibodies including those recognizing cytokeratins lymphoid markers S100 desmin and neurofilaments.

Homopolymer. Component of a complex composed at least of ACTB AP2M1 AP2A1 AP2A2 MEGF10 and VIM. Interacts with HCV core protein.

One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division at which time vimentin filaments are significantly reorganized.