Plant Total RNA Mini Kit (50prep)

centrifugation and vaccum

1. Make sure everything is RNase-free when handling RNA.
2. Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
3. Pipet a required volume of FARB Buffer or FAPRB Buffer to another RNase-free container and add 10 ul ß-mercaptoethanol
(ß-ME) per 1ml FARB Buffer or FAPRB Buffer before use. Caution: ß-mercaptoethanol is hazardous to human health. perform
the procedures involving FARB Buffer or FAPRB Buffer in a chemical fume hood.
4. Add required volume of RNase-free ethanol (96ca.100%) to Wash Buffer 2 when first use.
5. All centrifuge steps are done at full speed (ca.18, 000 x g) in a microcentrifuge.
6. Dilute RNase-free DNase 1 in reaction buffer (1M NaCl, 10 mM MnCl2, 20 mM Tris-HCl, pH 7.0 at 25C) to final conc. = 0.5 U/ul

30 ca. 60 minutes

up to 100 mg plant tissue or 1 x10 plant cells