Blood/Cultured Cell Total RNA Mini Kit (300prep)

up to 100 ug total RNA/ column

1. Make sure everything is RNase-free when handling RNA.
2. Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
3. Caution: ß-mercaptoethanol (ß-Me) is hazardous to human hea h. perform the procedures involving ß-Me in
a chemical fume hood.
4. Add required volume of RNase-free ethanol (96ca.100%) to Wash Buffer 2 when first use.
5. All centrifuge steps are done at full speed (ca.18, 000 x g) in a microcentrifuge.
6. Prepare RNase-free DNase 1 reaction buffer (1M NaCl, 10 mM MnCl2, 20 mM Tris-HCl, pH 7.0 at 25C)
and make the final concentration of DNase I to 0.5 U/ul.

30 ca. 60 minutes

Store at room temperature (15ca. 25 C) for 2 year.